100% USA Beef Trachea & Liver. Charki-O's are 100% natural, grain free healthy dog chews that are slow cooked to retain vital nutrients. Charki-O's are loaded with natural, stable Glucosamine & Chondroitin Sulphate with GAG's intact. 100% Raised, Harvested, Made and Packaged in the USA! Antibiotic Free and no added Hormones USA Beef Trachea and Liver. NO Preservatives, additives, antibiotics, hormones, fillers. NO JUNK! NO Glycerins, Glutens, Natural Flavorings, Grain. NO Phosphoric Acid, Sorbic Acid, Starch.